Within the BOW project HBM will participate in activities aimed at optimization of EV production, providing components such as EV depleted media, labelling reagents and protocols, and EV standards, as well as portable size focusing devices for SEC and TFF; Our team will contribute to comprehensive EV, and evMBDs characterization, and will ultimately verify and define minimal analytical package for definition of EV purity and quality (integrity, stability, and activity) to be assessed at each step of EV production scale up and engineering. Finally, HBM will perform in vitro tropism testing preceding definition of administration protocols for in vivo testing (doses and timeline)